I first noticed one spot on one of my middle sized clowns last Friday and having Protozin in the house immediately performed a water change before beginning treatment. The bottle of Protozin I used was one which was given to me when I bought my big tank and not being sure how fresh it was I decided to buy a new bottle on Saturday morning to continue treatment with.
The tank holds 350 L and taking decor etc. into account I decided to dose as if it held 300 L, but half the dose because of the loaches. So my dose was 10ml of Protozin. Following instructions on the bottle it should have been done on days 1,2,3 and then 6. Yesterday should have been day 4 and therefore no dose given, but I made the decision to dose for one more day because a) The initial dose was with the old protozin and b) instead of seeing any decrease in spots, there are a few more. The loaches are by no means riddled with them, we are talkng 3-4 visible spots on 2-3 of the clowns. So going by the life cycle of the whitespot my logic said - If these cysts lyse today then there would be no fresh medication in the water column to catch them. Anyway - that is the story so far. I don't know if it was an awful decision to make, but it made sense to me.
Since yesterday, my alpha loach seems very restless, otherwise fine. He/she is eating and very alert, but almost hyperactive, swimming up and down the tank - I take it they are becoming stressed. The only inhabitant of the tank who is visibly suffering is a golden pearlscale angelfish who is gasping and not eating.
I have a spraybar positioned above the waterline (its always like this) so that the water returning from the filter aerates the water as well as several airstones (3x and also an air disc) all along the back wall of the tank, I have dropped the water level as recommended and the temperature is at 27 C.
Water test performed 10 minutes ago using a Nutrafin test:
Ammonia 0
Nitrite 0
Nitrate 5
So, today the clowns are still restless, going round and round and up and down (mainly the alpha clown who has no visible spots).
The others have more spots about 6 spots each visible on 3 of them, the smallest clowns look like they have none. No spots are noticeable on any other fish in the tank (4x Angelfish, 1x sailfin plec, 10x neons and 10x penguin tetras plus 6 clowns in total)
Having decided to defy the instructions on the bottle last night

Should I do a water change to help calm the alpha loach? But then how would that affect the treatment?
I guess I have just lost the plot by trying to take it into my own hands

Can anyone give me some advice?
I do feel really silly for asking and please excuse the waffling too, I just wanted to tell the full story!
Thank-you