Slight information about DNA analysis of Stiphodon
Posted: Sat Feb 20, 2010 9:26 am
Hi plaalye and Matt.
I use the name of the "Rainbow-color", the "Blue-moon" and the "Orange-fin", but this doesn't mean the single species.
I shared with a group by the feature I tend to distinguish and just nicknamed those.
Each probably includes the species of the plural.
I insist repeatedly, but males of Stiphodon genus changes the color and the pattern drastically.
Therefore the condition that the species ID can be fixed only with one picture is limited.
I think as follows.
1. When there is a great difference beyond the range which can change.
2. When there is a clear difference in the part which doesn't change.
3. When the individual's habitat was the area where only the limited species is there.
4. When a DNA analysis result was identical with the standard specimen which can be trusted.
The pictures by which marketed Stiphodon is recognized as S.atratus and S.elegans are often come across.
But I have not seen a picture of S.atratus which can be trusted by a scientific field survey yet.
S.elegans can see several pictures in reports of the scientific field survey.
It seems different in shape of the first dorsal fin with "Ranbow-color".
http://www.li-an.fr/jyves/Talk_Meyer_Bi ... s_2003.pdf
http://www.bishopmuseum.org/research/pb ... report.pdf
http://calphotos.berkeley.edu/cgi/img_q ... on+elegans
http://w3.u-ryukyu.ac.jp/coe/action/new ... wsl10.html
Hi, Kajsa12.
The feature of that appearance is included in the range of the change in "Rainbow-color".
It wasn't possible to find the certain difference which can be classified only by the appearance.
It's because that's the individual in Palau for certain, that I judged the individual as S. pelewensis.
The progress of analysis technology of DNA sequence is remarkable.
An aquarist of hobbies would be able to request a DNA analysis by pocket money only by small sample of fish fin within 10 years now.
A DNA analysis result of just a little Stihodon is registered with a data base already.
When riches aids with a research fund, a DNA analysis of Stiphodon would be developed at a stroke.
Stiphodon elegans
http://www.ncbi.nlm.nih.gov/nuccore/162 ... rt=genbank
Stiphodon ornatus
http://www.ncbi.nlm.nih.gov/nuccore/126 ... rt=genbank
http://www.fishbase.org/Summary/Species ... p?ID=27467
for searching and analyzing genetic information (mitochondrial DNA) of Japanese freswater fishes.
http://gedimap.zool.kyoto-u.ac.jp/index ... a=2&lang=e
National Center for Biotechnology Information Databases
http://www.ncbi.nlm.nih.gov/sites/entrez
Dr. Mukai is telling a DNA analysis result of Stiphodon short separately in his site.
http://homepage2.nifty.com/PhD-mukai/La ... hodon.html
Before, he has shown the family tree of mitocondria DNA of Stiphodon he analyzed to a Japanese web forum.
There were no schedules which do academic release of his research result, so he showed that for us.
Unfortunately the thread isn't seen now.
I remember his research result.
The one which made simple is indicated here.
The original included a lot of individual's analysis results, but I made it simple.
The length of the branch of a crosswise direction is important to guess at a time, it's lost by my transcription.
━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━edited
┏━━━━━┫ Genus Sicyopterus
┃
┃━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━┏━━━━ F
┃━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━┏━━━━━┫ < "Orange-fin"
┃━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━┃━━━━━┗━━ M
┃━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━┃━━━━━━┏ S.pelewensis?(Guam)
┃━━━━━━━━━━━━━━━━━━━━━━━━━━┏━━━━━━┫━━━━━┏┫
┫━━━━━━━━━━━━━━━━━━━━━━━━━━┃━━━━━━┃┏━━━━┫┗━ S.pelewensis?(Palau)
┃━━━━━━━━━━━━━━━━━━━━━━━━━━┃━━━━━━┗┫━━━━┗━ <"Rainbow-color"
┃━━━━━━━━━━━━━━━━━━━┏━━━━━━┫━━━━━━━┗━━━━━━ S.imperiorientis
┃━━━━━━━━━━━━━━━━━━━┃━━━━━━┗━━━━━━━━━━━━━━ S.elegans
┃━━━━━━━━━━━━┏━━━━━━┫
┃━━━━━━━━━━━━┃━━━━━━┗━━━━━━━━━━━━━━━━ ? S.alcedo ?
┃━━━━━┏━━━━━━┫
┃━━━━━┃━━━━━━┃━━━━━━━━━━━━━・・・・・< "Blue-moon"
┃━━━━━┃━━━━━━┗━━━━━━━━━━━━━━━━━━━━━ S.atropurpureus
┃━━━━━┃
┗━━━━━┫ Genus Stiphodon
━━━━━━┗━━━━━━━━━━━━━━━━━━━━━━━━━━ S.percnopterygionus
I use the name of the "Rainbow-color", the "Blue-moon" and the "Orange-fin", but this doesn't mean the single species.
I shared with a group by the feature I tend to distinguish and just nicknamed those.
Each probably includes the species of the plural.
I insist repeatedly, but males of Stiphodon genus changes the color and the pattern drastically.
Therefore the condition that the species ID can be fixed only with one picture is limited.
I think as follows.
1. When there is a great difference beyond the range which can change.
2. When there is a clear difference in the part which doesn't change.
3. When the individual's habitat was the area where only the limited species is there.
4. When a DNA analysis result was identical with the standard specimen which can be trusted.
The pictures by which marketed Stiphodon is recognized as S.atratus and S.elegans are often come across.
But I have not seen a picture of S.atratus which can be trusted by a scientific field survey yet.
S.elegans can see several pictures in reports of the scientific field survey.
It seems different in shape of the first dorsal fin with "Ranbow-color".
http://www.li-an.fr/jyves/Talk_Meyer_Bi ... s_2003.pdf
http://www.bishopmuseum.org/research/pb ... report.pdf
http://calphotos.berkeley.edu/cgi/img_q ... on+elegans
http://w3.u-ryukyu.ac.jp/coe/action/new ... wsl10.html
Hi, Kajsa12.
The feature of that appearance is included in the range of the change in "Rainbow-color".
It wasn't possible to find the certain difference which can be classified only by the appearance.
It's because that's the individual in Palau for certain, that I judged the individual as S. pelewensis.
The progress of analysis technology of DNA sequence is remarkable.
An aquarist of hobbies would be able to request a DNA analysis by pocket money only by small sample of fish fin within 10 years now.
A DNA analysis result of just a little Stihodon is registered with a data base already.
When riches aids with a research fund, a DNA analysis of Stiphodon would be developed at a stroke.
Stiphodon elegans
http://www.ncbi.nlm.nih.gov/nuccore/162 ... rt=genbank
Stiphodon ornatus
http://www.ncbi.nlm.nih.gov/nuccore/126 ... rt=genbank
http://www.fishbase.org/Summary/Species ... p?ID=27467
for searching and analyzing genetic information (mitochondrial DNA) of Japanese freswater fishes.
http://gedimap.zool.kyoto-u.ac.jp/index ... a=2&lang=e
National Center for Biotechnology Information Databases
http://www.ncbi.nlm.nih.gov/sites/entrez
Dr. Mukai is telling a DNA analysis result of Stiphodon short separately in his site.
http://homepage2.nifty.com/PhD-mukai/La ... hodon.html
Before, he has shown the family tree of mitocondria DNA of Stiphodon he analyzed to a Japanese web forum.
There were no schedules which do academic release of his research result, so he showed that for us.
Unfortunately the thread isn't seen now.
I remember his research result.
The one which made simple is indicated here.
The original included a lot of individual's analysis results, but I made it simple.
The length of the branch of a crosswise direction is important to guess at a time, it's lost by my transcription.
━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━edited
┏━━━━━┫ Genus Sicyopterus
┃
┃━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━┏━━━━ F
┃━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━┏━━━━━┫ < "Orange-fin"
┃━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━┃━━━━━┗━━ M
┃━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━┃━━━━━━┏ S.pelewensis?(Guam)
┃━━━━━━━━━━━━━━━━━━━━━━━━━━┏━━━━━━┫━━━━━┏┫
┫━━━━━━━━━━━━━━━━━━━━━━━━━━┃━━━━━━┃┏━━━━┫┗━ S.pelewensis?(Palau)
┃━━━━━━━━━━━━━━━━━━━━━━━━━━┃━━━━━━┗┫━━━━┗━ <"Rainbow-color"
┃━━━━━━━━━━━━━━━━━━━┏━━━━━━┫━━━━━━━┗━━━━━━ S.imperiorientis
┃━━━━━━━━━━━━━━━━━━━┃━━━━━━┗━━━━━━━━━━━━━━ S.elegans
┃━━━━━━━━━━━━┏━━━━━━┫
┃━━━━━━━━━━━━┃━━━━━━┗━━━━━━━━━━━━━━━━ ? S.alcedo ?
┃━━━━━┏━━━━━━┫
┃━━━━━┃━━━━━━┃━━━━━━━━━━━━━・・・・・< "Blue-moon"
┃━━━━━┃━━━━━━┗━━━━━━━━━━━━━━━━━━━━━ S.atropurpureus
┃━━━━━┃
┗━━━━━┫ Genus Stiphodon
━━━━━━┗━━━━━━━━━━━━━━━━━━━━━━━━━━ S.percnopterygionus















